Journal: STAR Protocols

Article Title: Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

doi: 10.1016/j.xpro.2021.100971

Figure Lengend Snippet:

Article Snippet: Guinea pig polyclonal anti-Calbindin (1:100) , Synaptic Systems , Cat#214 005.

Techniques: Recombinant, Saline, Electron Microscopy, Plasmid Preparation, Software, Imaging, Control, Mass Measurement, Capsules, Microscopy, Staining, Adhesive, Spectrophotometry

Journal: The Journal of comparative neurology

Article Title: Differential roles of ventral pallidum subregions during cocaine self-administration behaviors

doi: 10.1002/cne.23191

Figure Lengend Snippet: Primary Antibodies Used For further details regarding the primary antibodies, see the Antibody Characterization section

Article Snippet: Free-floating sections were rinsed in 0.1 M phosphate buffer (pH 7.4), placed into 1% sodium borohydride for 15 min, thoroughly rinsed in 0.1 M phosphate buffer again, pretreated with 0.1 M phosphate buffer containing 0.1% Triton X-100 and 3% normal goat serum for 1 h, and then transferred into a solution containing a primary antibody, either polyclonal anti-neurotensin (ImmunoStar, Inc., Hudson, WI; formerly DiaSorin Histochemical as well as INCSTAR; catalog number 20072) at a dilution of 1: 6500, polyclonal anti-calbindin-d28k (ImmunoStar; catalog number 24427) at a dilution of 1: 6000, or polyclonal anti-substance P (ImmunoStar catalog number 20064) at 1: 6500 in 0.1 M phosphate buffer with 0.1% Triton X-100 and 3% normal goat serum overnight (see and Antibody Characterization section for details regarding the primary antibodies used).

Techniques: Staining, Sequencing, Purification

Journal: The Journal of comparative neurology

Article Title: Differential roles of ventral pallidum subregions during cocaine self-administration behaviors

doi: 10.1002/cne.23191

Figure Lengend Snippet: Example microwires localized to VP subregions. Examples are displayed in groups of three. A-I displays three examples of microwires localized to the VPdl in three vertical panels each; example one A-C, example two D-F, and example three G-I. J-R display three examples of microwires localized to the VPvm in three vertical panels each; example one J-L, example two M-O, and example three P-R. Panels A, D, G, J, M, and P display substance P immunohistochemistry. Panels B, E, H, K, N, and Q display calbindin-d28k immunohistochemistry. Panels C, F, I, L, O, and R display neurotensin immunohistochemistry. Green/blue dot in each panel is an iron deposit from the uninsulated microwire tip visualized from by potassium ferrocyanide counterstain. Numbers refer to approximate anteroposterior coordinate based on Paxinos and Watson (2004). As a model for all VPdl neurons recorded, VPdl example 1 (A-C) displays two microwire tip locations. The wire closest to the anterior commissure was localized to substance P immunoreactivity (A) and calbindin-d28k immunoreactivity (B), but not neurotensin immunoreactivity (C). The microwire furthest from the anterior commissure (A) was localized outside of substance P immunoreactivity and therefore excluded from the dataset. As a model for all VPvm neurons recorded, VPvm example 1 (J-L) displays a microwire tip location localized to substance P immunoreactivity (J) and neurotensin immunoreactivity (K), but not calbindin-d28k immunoreactivity (L). Any tissue from the left hemisphere has been rotated horizontally to the right hemisphere for the sake of uniform orientation (midline is left for all panels). Outlines were based on substance P, calbindin-d28k, and neurotensin immunoreactivity, the atlas of Paxinos and Watson (2004), and scientific literature (Zahm and Heimer, 1988, 1990; Zahm 1989; Zahm et al. 1996; Riedel et al. 2002; Tripathi et al. 2010). Calibration bar in A represents 1 mm. All sections were 40 μm thick.

Article Snippet: Free-floating sections were rinsed in 0.1 M phosphate buffer (pH 7.4), placed into 1% sodium borohydride for 15 min, thoroughly rinsed in 0.1 M phosphate buffer again, pretreated with 0.1 M phosphate buffer containing 0.1% Triton X-100 and 3% normal goat serum for 1 h, and then transferred into a solution containing a primary antibody, either polyclonal anti-neurotensin (ImmunoStar, Inc., Hudson, WI; formerly DiaSorin Histochemical as well as INCSTAR; catalog number 20072) at a dilution of 1: 6500, polyclonal anti-calbindin-d28k (ImmunoStar; catalog number 24427) at a dilution of 1: 6000, or polyclonal anti-substance P (ImmunoStar catalog number 20064) at 1: 6500 in 0.1 M phosphate buffer with 0.1% Triton X-100 and 3% normal goat serum overnight (see and Antibody Characterization section for details regarding the primary antibodies used).

Techniques: Immunohistochemistry

Journal: The Journal of Neuroscience

Article Title: Sex-Dependent Regulation of Aromatase-Mediated Synaptic Plasticity in the Basolateral Amygdala

doi: 10.1523/JNEUROSCI.1532-16.2016

Figure Lengend Snippet: AROM expression in major subregions of rodent amygdala. A, Coronal section through anterior amygdala of an young adult mouse, immunostained for AROM using rabbit polyclonal antibodies (Yague et al., 2006). Substantial AROM expression is detectable in the MeA and CeA, in the BL and La nucleus of the BLA, and in the adjacent piriform cortex (Pir). Virtually, no AROM immunoreactivity is seen in the BM of BLA. Low levels of expression were found in the cortical amygdala (CoA). Scale bar, 250 μm. B, Western blots showing AROM protein expression in amygdala subregions CeA, BLA, and MeA of juvenile male and female rats using monoclonal antibodies against AROM (Acris). Tissue from hippocampus (Hip), cerebellum (Cer), and somatosensory cortex (Cor) was blotted for comparison. The strongest signal is found in the MeA. AROM expression levels in the CeA and BLA are comparable to levels in the hippocampus, neocortex, and cerebellum (note: a representative band for the female CeA was inserted from a different gel). C, Quantitative analysis of Western blot data, comparing AROM expression in BLA of age-matched (P20–P24) juvenile male and female rats. No difference between the sexes was evident (rel. expression of AROM: 0.46 ± 0.06 in females; 0.48 ± 0.09 in males; p = 0.88; n = 8 of each sex).

Article Snippet: Rabbit polyclonal anti-calbindin D28K antibodies (Calb; 1:2000; Merck Millipore, Billerica, MA, USA, Cat# AB1778, RRID:AB_2068336) were applied for characterization of the cultures.

Techniques: Expressing, Western Blot, Bioprocessing, Comparison